Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats

The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbi...

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Main Authors: Hussin, Farah Salina, Chay, Shyan Yea, Zarei, Mohammad, Meor Hussin, Anis Shobirin, Wan Ibadullah, Wan Zunairah, Zaharuddin, Nurul Dhania, Wazir, Hazrati, Saari, Nazamid
Format: Article
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020
Online Access:http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf
http://psasir.upm.edu.my/id/eprint/87231/
https://www.mdpi.com/2304-8158/9/12/1826
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spelling my.upm.eprints.872312022-01-20T04:32:32Z http://psasir.upm.edu.my/id/eprint/87231/ Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats Hussin, Farah Salina Chay, Shyan Yea Zarei, Mohammad Meor Hussin, Anis Shobirin Wan Ibadullah, Wan Zunairah Zaharuddin, Nurul Dhania Wazir, Hazrati Saari, Nazamid The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbiotically revealed the most prominent GABA-producing effect. Response surface methodology revealed the optimized fermentation conditions at 39.0 °C, 7.25 h, and 11.5 mM glutamate substrate concentration to produce GABA-rich yogurt (29.96 mg/100 g) with desirable pH (3.93) and water-holding capacity (63.06%). At 2% glucose to replace pyridoxal-5-phosphate (PLP), a cofactor typically needed during GABA production, GABA content was further enhanced to 59.00 mg/100 g. In vivo study using this sample revealed a blood pressure-lowering efficacy at 0.1 mg/kg GABA dosage (equivalent to 30 mg/kg GABA-rich yogurt) in spontaneously hypertensive rats. An improved method to produce GABA-rich yogurt has been established, involving shorter fermentation time and lower glutamate concentration than previous work, along with glucose induction that omits the use of costly PLP, fostering the potential of developing a GABA-rich functional dairy product through natural fermentation with desirable product quality and antihypertensive property. Multidisciplinary Digital Publishing Institute 2020-12-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf Hussin, Farah Salina and Chay, Shyan Yea and Zarei, Mohammad and Meor Hussin, Anis Shobirin and Wan Ibadullah, Wan Zunairah and Zaharuddin, Nurul Dhania and Wazir, Hazrati and Saari, Nazamid (2020) Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats. Foods, 9 (12). pp. 1-15. ISSN 2304-8158 https://www.mdpi.com/2304-8158/9/12/1826 10.3390/foods9121826
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The current study evaluated the γ-aminobutyric acid (GABA) producing ability from three novel strains of lactic acid bacteria (L. plantarum Taj-Apis362, assigned as UPMC90, UPMC91, and UPMC1065) co-cultured with starter culture in a yogurt. A combination of UPMC90 + UPMC91 with starter culture symbiotically revealed the most prominent GABA-producing effect. Response surface methodology revealed the optimized fermentation conditions at 39.0 °C, 7.25 h, and 11.5 mM glutamate substrate concentration to produce GABA-rich yogurt (29.96 mg/100 g) with desirable pH (3.93) and water-holding capacity (63.06%). At 2% glucose to replace pyridoxal-5-phosphate (PLP), a cofactor typically needed during GABA production, GABA content was further enhanced to 59.00 mg/100 g. In vivo study using this sample revealed a blood pressure-lowering efficacy at 0.1 mg/kg GABA dosage (equivalent to 30 mg/kg GABA-rich yogurt) in spontaneously hypertensive rats. An improved method to produce GABA-rich yogurt has been established, involving shorter fermentation time and lower glutamate concentration than previous work, along with glucose induction that omits the use of costly PLP, fostering the potential of developing a GABA-rich functional dairy product through natural fermentation with desirable product quality and antihypertensive property.
format Article
author Hussin, Farah Salina
Chay, Shyan Yea
Zarei, Mohammad
Meor Hussin, Anis Shobirin
Wan Ibadullah, Wan Zunairah
Zaharuddin, Nurul Dhania
Wazir, Hazrati
Saari, Nazamid
spellingShingle Hussin, Farah Salina
Chay, Shyan Yea
Zarei, Mohammad
Meor Hussin, Anis Shobirin
Wan Ibadullah, Wan Zunairah
Zaharuddin, Nurul Dhania
Wazir, Hazrati
Saari, Nazamid
Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
author_facet Hussin, Farah Salina
Chay, Shyan Yea
Zarei, Mohammad
Meor Hussin, Anis Shobirin
Wan Ibadullah, Wan Zunairah
Zaharuddin, Nurul Dhania
Wazir, Hazrati
Saari, Nazamid
author_sort Hussin, Farah Salina
title Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
title_short Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
title_full Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
title_fullStr Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
title_full_unstemmed Potentiality of self-cloned Lactobacillus plantarum Taj-Apis362 for enhancing GABA production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
title_sort potentiality of self-cloned lactobacillus plantarum taj-apis362 for enhancing gaba production in yogurt under glucose induction: optimization and its cardiovascular effect on spontaneous hypertensive rats
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020
url http://psasir.upm.edu.my/id/eprint/87231/1/Potentiality%20of%20self-cloned%20Lactobacillus.pdf
http://psasir.upm.edu.my/id/eprint/87231/
https://www.mdpi.com/2304-8158/9/12/1826
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score 13.209306