Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology

• Main Authors: Kusuma, Sri Agung Fitri, Parwati, Ida, Rostinawati, Tina, Yusuf, Muhammad, Fadhlillah, Muhammad, Ahyudanari, Risa R., Rukayadi, Yaya, Subroto, Toto
• Format: Article
• Language: English
• Published: Elsevier 2019
• Online Access: http://psasir.upm.edu.my/id/eprint/81564/1/Optimization%20of%20culture%20conditions%20for%20Mpt64%20synthetic%20gene%20expression%20in%20Escherichia%20coli%20BL21%20%28DE3%29%20using%20surface%20response%20methodology.pdf; http://psasir.upm.edu.my/id/eprint/81564/; https://www.sciencedirect.com/science/article/pii/S2405844019364011#!

MPT64 is a specific protein that is secreted by Mycobacterium tuberculosis complex (MTBC). The objective of this study was to obtain optimum culture conditions for MPT64 synthetic gene expression in Escherichia coli BL21 (DE3) by response surface methodology (RSM). The RSM was undertaken to optimize...