Construction and expression of synthetic gene encoding MPT64 as extracellular protein in Escherichia coli BL21 (DE3) expression system

• Main Authors: Kusuma, Sri Agung Fitri, Ida Parwati, Tina Rostinawati, Muhammad Yusuf, Muhammad Fadhlillah, Laily D. Tanti, Ahyudanari, Risa R., Rukayadi, Yaya, Subroto, Toto
• Format: Article
• Language: English
• Published: Pharmainfo Publications 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/72109/1/Construction%20and%20expression%20of%20synthetic%20gene%20encoding%20MPT64%20as%20extracellular%20protein%20in%20Escherichia%20coli%20BL21%20%28DE3%29%20expression%20system.pdf; http://psasir.upm.edu.my/id/eprint/72109/; https://www.jpsr.pharmainfo.in/issue.php?page=110#

Aim: This study was purposed to produce MPT64 protein extracellularly by contructing pelB signal peptide into expression vector containing synthetic gene of MPT64 from Mycobacterium tuberculosis H37Rv Methods: The mpt64 synthetic gene was derived from gene sequence of M. tuberculosis H37Rv. Therefor...