The effects of one amino acid substitutions at the C-terminal region of thermostable L2 lipase by computational and experimental approach

• Main Authors: Ahmad Sani, Hartini, Mohd Shariff, Fairolniza, Raja Abdul Rahman, Raja Noor Zaliha, Thean, Chor Leow, Salleh, Abu Bakar
• Format: Article
• Language: English
• Published: Springer 2018
• Online Access: http://psasir.upm.edu.my/id/eprint/74173/1/The%20effects%20of%20one%20amino%20acid%20.pdf; http://psasir.upm.edu.my/id/eprint/74173/; https://link.springer.com/article/10.1007%2Fs12033-017-0038-3

The substitutions of the amino acid at the predetermined critical point at the C-terminal of L2 lipase may increase its thermostability and enzymatic activity, or even otherwise speed up the unfolding of the protein structure. The C-terminal of most proteins is often flexible and disordered. However...