Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation
Introduction: Airway inflammation is the pathological hallmark of chronic inflammatory airway diseases, especially asthma and chronic obstructive pulmonary disease (COPD). Airway epithelium plays an indispensable role in these diseases by secreting inflammatory mediators and cytokines in response to...
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2019
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my.upm.eprints.700242019-08-16T00:38:38Z http://psasir.upm.edu.my/id/eprint/70024/ Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation Liew, Kong Yen Rosly, Nur A'dilah Abas, Faridah Leong, Sze Wei Israf Ali, Daud Ahmad Tham, Chau Ling Introduction: Airway inflammation is the pathological hallmark of chronic inflammatory airway diseases, especially asthma and chronic obstructive pulmonary disease (COPD). Airway epithelium plays an indispensable role in these diseases by secreting inflammatory mediators and cytokines in response to foreign substances, such as lipopolysaccharide (LPS). Previous studies have shown that diarylpentanoid analogues, especially 5-(3,4-dihydroxyphenyl)-3-hy-droxy-1-(2-hydroxyphenyl)penta-2,4-dien-1-one (DHHPD) and 2-benzoyl-6-(3,4-dihydroxybenzylidene)cyclohex-en-1-ol (BDHBC), significantly inhibited nitric oxide (NO) production; suggesting their anti-inflammatory property. However, the therapeutic potential of DHHPD and BDHBC in airway inflammation has not been explored. Thus, this study aims to investigate their effects on interleukin (IL)-6 and IL-8 gene expression in LPS-induced Calu-3 cells, a cellular model of human airway epithelium. Methods: MTT cytotoxicity assay was carried out to identify non-cytotoxic concentrations of DHHPD and BDHBC on Calu-3 cells. RT-PCR was done to determine IL-6 and IL-8 gene expression levels. Results: DHHPD and BDHBC were not cytotoxic on Calu-3 cells up to 200μM. Four non-cytotoxic concentrations were chosen – 6.25, 12.5, 25 and 50μM to determine the effect of both compounds on gene expression. All four concentrations of DHHPD and BDHBC significantly inhibited LPS-induced mRNA expression of IL-6 while all concentrations of BDHBC, except 6.25μM, significantly reduced IL-8 mRNA expression. Similar finding was obtained for DHHPD, except that at 50μM, there was no inhibition of IL-8 mRNA expression. Conclusion: Diarylpentanoid analogues, DHHPD and BDHBC, are proven to be effective in suppressing LPS-induced IL-6 and IL-8 gene expression. However, further studies are required to confirm their inhibitory effects on the production of pro-inflammatory cytokines. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/70024/1/2019070209582102_MJMHS_SP2_2019.pdf Liew, Kong Yen and Rosly, Nur A'dilah and Abas, Faridah and Leong, Sze Wei and Israf Ali, Daud Ahmad and Tham, Chau Ling (2019) Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation. Malaysian Journal of Medicine and Health Sciences, 15 (SP2). pp. 10-17. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2019070209582102_MJMHS_SP2_2019.pdf |
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Introduction: Airway inflammation is the pathological hallmark of chronic inflammatory airway diseases, especially asthma and chronic obstructive pulmonary disease (COPD). Airway epithelium plays an indispensable role in these diseases by secreting inflammatory mediators and cytokines in response to foreign substances, such as lipopolysaccharide (LPS). Previous studies have shown that diarylpentanoid analogues, especially 5-(3,4-dihydroxyphenyl)-3-hy-droxy-1-(2-hydroxyphenyl)penta-2,4-dien-1-one (DHHPD) and 2-benzoyl-6-(3,4-dihydroxybenzylidene)cyclohex-en-1-ol (BDHBC), significantly inhibited nitric oxide (NO) production; suggesting their anti-inflammatory property. However, the therapeutic potential of DHHPD and BDHBC in airway inflammation has not been explored. Thus, this study aims to investigate their effects on interleukin (IL)-6 and IL-8 gene expression in LPS-induced Calu-3 cells, a cellular model of human airway epithelium. Methods: MTT cytotoxicity assay was carried out to identify non-cytotoxic concentrations of DHHPD and BDHBC on Calu-3 cells. RT-PCR was done to determine IL-6 and IL-8 gene expression levels. Results: DHHPD and BDHBC were not cytotoxic on Calu-3 cells up to 200μM. Four non-cytotoxic concentrations were chosen – 6.25, 12.5, 25 and 50μM to determine the effect of both compounds on gene expression. All four concentrations of DHHPD and BDHBC significantly inhibited LPS-induced mRNA expression of IL-6 while all concentrations of BDHBC, except 6.25μM, significantly reduced IL-8 mRNA expression. Similar finding was obtained for DHHPD, except that at 50μM, there was no inhibition of IL-8 mRNA expression. Conclusion: Diarylpentanoid analogues, DHHPD and BDHBC, are proven to be effective in suppressing LPS-induced IL-6 and IL-8 gene expression. However, further studies are required to confirm their inhibitory effects on the production of pro-inflammatory cytokines. |
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Article |
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Liew, Kong Yen Rosly, Nur A'dilah Abas, Faridah Leong, Sze Wei Israf Ali, Daud Ahmad Tham, Chau Ling |
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Liew, Kong Yen Rosly, Nur A'dilah Abas, Faridah Leong, Sze Wei Israf Ali, Daud Ahmad Tham, Chau Ling Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
author_facet |
Liew, Kong Yen Rosly, Nur A'dilah Abas, Faridah Leong, Sze Wei Israf Ali, Daud Ahmad Tham, Chau Ling |
author_sort |
Liew, Kong Yen |
title |
Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
title_short |
Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
title_full |
Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
title_fullStr |
Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
title_full_unstemmed |
Effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
title_sort |
effect of diarylpentanoid analogues on lipopolysaccharide-induced interleukin-6 and interleukin-8 gene expression in airway inflammation |
publisher |
Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
publishDate |
2019 |
url |
http://psasir.upm.edu.my/id/eprint/70024/1/2019070209582102_MJMHS_SP2_2019.pdf http://psasir.upm.edu.my/id/eprint/70024/ https://medic.upm.edu.my/upload/dokumen/2019070209582102_MJMHS_SP2_2019.pdf |
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13.18916 |