The Evaluation, Production, and Derivatisation of the Garcinia Acid in Garcinia Atroviridis
A method for extraction and isolation of garcinia acid from Garcinia atroviridis (G.atr0viridi.s) was developed. Using this method high yields of garcinia acid in the range of 90-95 g from 500 g of the dried rinds were accomplished. This method is low cost and simple. The garcinia acid isolated f...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2005
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/6768/1/IB_2005_10.pdf http://psasir.upm.edu.my/id/eprint/6768/ |
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| Summary: | A method for extraction and isolation of garcinia acid from Garcinia atroviridis
(G.atr0viridi.s) was developed. Using this method high yields of garcinia acid in the
range of 90-95 g from 500 g of the dried rinds were accomplished. This method is low
cost and simple. The garcinia acid isolated from this procedure was converted to its
calcium salt of HCA lactone to ensure that the compound was stable and less
hygroscopic. From the analysis using high performance liquid chromatography (HPLC),
three peaks of organic acids were observed and identified as a-hydroxycitric acid
lactone (HCA lactone), a-hydroxycitric acid (HCA) and citric acid based on spiking
with standard acids and retention time. The amount of the HCA was 72 % wlw, the
HCA lactone was 17 % wlw and the citric acid was 0.4 % wlw in the calcium salt of
HCA lactone. The calcium salt of HCA lactone was derivatised into ester. The
esterification products are dimethyl tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate
and diethyl tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylateB. oth esters were tested
for biological activity towards nitric oxide (NO). Unfortunately, both compounds were found inactive towards NO inhibition RAW 264.7 cell treated with lipopolysaccharide
(LPS) and interferon-y (IFN-y). |
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