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Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea

An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN),...

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Main Authors: Niknejad, Azadeh, Abdul Kadir, Mihdzar, Kadzimin, Saleh, Abdullah, N. A.
Format: Conference or Workshop Item
Language:English
Published: Sarawak Biodiversity Centre 2008
Online Access:http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf
http://psasir.upm.edu.my/id/eprint/64277/
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spelling my.upm.eprints.642772018-07-04T02:31:37Z http://psasir.upm.edu.my/id/eprint/64277/ Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Abdullah, N. A. An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Abdullah, N. A. (2008) Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 428-431).
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs.
format Conference or Workshop Item
author Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Abdullah, N. A.
spellingShingle Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Abdullah, N. A.
Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
author_facet Niknejad, Azadeh
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Abdullah, N. A.
author_sort Niknejad, Azadeh
title Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
title_short Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
title_full Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
title_fullStr Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
title_full_unstemmed Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
title_sort induction of callus and protocorm-like bodies (plbs) in phalaenopsis gigantea
publisher Sarawak Biodiversity Centre
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf
http://psasir.upm.edu.my/id/eprint/64277/
_version_ 1643837976777588736
score 13.211869

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