Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN),...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Sarawak Biodiversity Centre
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf http://psasir.upm.edu.my/id/eprint/64277/ |
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| Summary: | An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs. |
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