Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus
A SYBR Green I based one-step real-time reverse transcriptase polymerase chain reaction was developed for the detection and differentiation of very virulent (vv) and classical strains of infectious bursal disease virus (IBDV). The assay showed high PCR efficiency >93% and high reproducibility w...
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Main Authors: | Lih, Ling Kong, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Tan, Sheau Wei |
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Format: | Article |
Language: | English English |
Published: |
Elsevier
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/6338/1/Development%20of%20SYBR%20green%20I%20based%20one.pdf http://psasir.upm.edu.my/id/eprint/6338/ http://dx.doi.org/10.1016/j.jviromet.2009.06.023 |
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