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Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus

A SYBR Green I based one-step real-time reverse transcriptase polymerase chain reaction was developed for the detection and differentiation of very virulent (vv) and classical strains of infectious bursal disease virus (IBDV). The assay showed high PCR efficiency >93% and high reproducibility w...

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Bibliographic Details
Main Authors: Lih, Ling Kong, Omar, Abdul Rahman, Bejo, Mohd Hair, Ideris, Aini, Tan, Sheau Wei
Format: Article
Language:English
English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/6338/1/Development%20of%20SYBR%20green%20I%20based%20one.pdf
http://psasir.upm.edu.my/id/eprint/6338/
http://dx.doi.org/10.1016/j.jviromet.2009.06.023
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http://psasir.upm.edu.my/id/eprint/6338/1/Development%20of%20SYBR%20green%20I%20based%20one.pdf
http://psasir.upm.edu.my/id/eprint/6338/
http://dx.doi.org/10.1016/j.jviromet.2009.06.023

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