Physical and biological parameters affecting transient GUS and GFP expression in banana via particle bombardment

• Main Authors: Subramaniam, Sreeramanan, Mahmood, Maziah, Abdullah, Mohd Puad, Meon, Sariah, Xavier, Rathinam, Mohd Fadzillah, Nor'aini
• Format: Article
• Language: English
• Published: Malaysian Society for Molecular Biology and Biotechnology 2005
• Online Access: http://psasir.upm.edu.my/id/eprint/58489/1/Physical%20and%20biological%20parameters%20affecting%20transient%20GUS%20and%20GFP%20expression%20in%20banana%20via%20particle%20bombardment.pdf; http://psasir.upm.edu.my/id/eprint/58489/; http://www.msmbb.org.my/apjmbb/html13/13d.htm

Physical and biological parameters for DNA delivery into banana cultivar, Rastali (AAB) explants were optimised by monitoring transient gusA and gfp gene expression. Optimisation of the physical factors was carried out under the following conditions; helium pressure (450, 900, 1100, 1350 and 1550psi...