Cold-adapted organic solvent tolerant alkalophilic family I.3 lipase from an Antarctic Pseudomonas

• Main Authors: Ganasen, Menega, Yaacob, Norhayati, Raja Abd Rahman, Raja Noor Zaliha, Thean, Adam Chor Leow, Basri, Mahiran, Salleh, Abu Bakar, Mohamad Ali, Mohd Shukuri
• Format: Article
• Language: English
• Published: Elsevier BV 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/55306/1/Cold-adapted%20organic%20solvent%20tolerant%20alkalophilic%20family%20I.3%20lipase%20from%20an%20Antarctic%20.pdf; http://psasir.upm.edu.my/id/eprint/55306/

Lipolytic enzymes with cold adaptation are gaining increasing interest due to their biotechnological prospective. Previously, a cold adapted family I.3 lipase (AMS8 lipase) was isolated from an Antarctic Pseudomonas. AMS8 lipase was largely expressed in insoluble form. The refolded His-tagged recomb...