Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3

• Main Authors: Latip, Wahhida, Raja Abd Rahman, Raja Noor Zaliha, Thean, Adam Chor Leow, Mohd Shariff, Fairolniza, Mohamad Ali, Mohd Shukuri
• Format: Article
• Language: English
• Published: PeerJ 2016
• Online Access: http://psasir.upm.edu.my/id/eprint/54245/1/Expression%20and%20characterization%20of.pdf; http://psasir.upm.edu.my/id/eprint/54245/; https://peerj.com/articles/2420/

A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10-70 °C and stable over a broad pH range from 5.0 to pH 10.0. Various mono and divalent metal ions increased the activity of the AMS3 lipase, but Ni2+ decreased its activity. The purified lipase exhibited the highest activity in the presence of sunflower oil. In addition, the enzyme activity in 25% v/v solvents at 50 °C particularly to n-hexane, DMSO and methanol could be useful for catalysis reaction in organic solvent and at broad temperature.