Effect of elicitors on the production of naringin and rutin in leech lime (Citrus hystrix) callus

• Main Authors: Roowi, Suri, Muse, Radzali, Mahmood, Marziah, Sukari, Mohd Aspollah
• Format: Article
• Language: English
• Published: Malaysian Agricultural Research and Development Institute (MARDI) 2002
• Online Access: http://psasir.upm.edu.my/id/eprint/48930/1/Effect%20of%20elicitors%20on%20the%20production%20of%20naringin%20and%20rutin%20in%20leech%20lime%20%28Citrus%20hystrix%29%20callus.pdf; http://psasir.upm.edu.my/id/eprint/48930/; http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/80-2002/volume-30-no1/302-300102

Treatment of Citrus hystrix callus with various types of elicitors decreased the callus growth as the concentration of elicitors increased. However, callus growth remained relatively constant at 0.05% (w/v) alginate and slightly increased to 1.30 ± 0.04 g dwt./culture at 0.1% (w/v) alginate. Analysis of the flavonoids using high performance liquid chromatography (HPLC) showed that only naringin and rutin were produced. Maximum production of naringin (12.13 ± 0.07 mg/g dwt.) and rutin (3.09 ± 0.05 mg/g dwt.) (p ≤ 0.05) was found in callus treated with 0.5% (w/v) agarose.