The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis
The toxin-antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA s...
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Dove Medical Press
2015
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Online Access: | http://psasir.upm.edu.my/id/eprint/46648/1/The%20mazef%20toxin-antitoxin%20system%20as%20an%20attractive%20target%20in%20clinical%20isolates%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis.pdf http://psasir.upm.edu.my/id/eprint/46648/ http://www.dovepress.com/drug-design-development-and-therapy-journal |
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my.upm.eprints.466482018-02-22T08:45:13Z http://psasir.upm.edu.my/id/eprint/46648/ The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis Soheili, Sara Ghafourian, Sobhan Sekawi, Zamberi Neela, Vasantha Kumari Sadeghifard, Nourkhoda Taherikalani, Morovat Khosravi, Afra Ramli, Ramliza Awang Hamat, Rukman The toxin-antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems are potent and sensitive targets in all E. faecium and E. faecalis strains. Dove Medical Press 2015-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/46648/1/The%20mazef%20toxin-antitoxin%20system%20as%20an%20attractive%20target%20in%20clinical%20isolates%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis.pdf Soheili, Sara and Ghafourian, Sobhan and Sekawi, Zamberi and Neela, Vasantha Kumari and Sadeghifard, Nourkhoda and Taherikalani, Morovat and Khosravi, Afra and Ramli, Ramliza and Awang Hamat, Rukman (2015) The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis. Drug Design, Development and Therapy, 9. pp. 2553-2561. ISSN 1177-8881 http://www.dovepress.com/drug-design-development-and-therapy-journal 10.2147/DDDT.S77263 |
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The toxin-antitoxin (TA) system is a regulatory system where two sets of genes encode the toxin and its corresponding antitoxin. In this study, the prevalence of TA systems in independently isolated clinical isolates of Enterococcus faecium and Enterococcus faecalis was determined, the dominant TA system was identified, different virulence genes in E. faecium and E. faecalis were surveyed, the level of expression of the virulence and TA genes in normal and stress conditions was determined, and finally their associations with the TA genes were defined. Remarkably, the analysis demonstrated higBA and mazEF in all clinical isolates, and their locations were on chromosomes and plasmids, respectively. On the other hand, a quantitative analysis of TA and virulence genes revealed that the expression level in both genes is different under normal and stress conditions. The results obtained by anti-mazF peptide nucleic acids demonstrated that the expression level of virulence genes had decreased. These findings demonstrate an association between TA systems and virulence factors. The mazEF on the plasmids and the higBA TA genes on the chromosomes of all E. faecium and E. faecalis strains were dominant. Additionally, there was a decrease in the expression of virulence genes in the presence of anti-mazF peptide nucleic acids. Therefore, it is suggested that mazEF TA systems are potent and sensitive targets in all E. faecium and E. faecalis strains. |
format |
Article |
author |
Soheili, Sara Ghafourian, Sobhan Sekawi, Zamberi Neela, Vasantha Kumari Sadeghifard, Nourkhoda Taherikalani, Morovat Khosravi, Afra Ramli, Ramliza Awang Hamat, Rukman |
spellingShingle |
Soheili, Sara Ghafourian, Sobhan Sekawi, Zamberi Neela, Vasantha Kumari Sadeghifard, Nourkhoda Taherikalani, Morovat Khosravi, Afra Ramli, Ramliza Awang Hamat, Rukman The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
author_facet |
Soheili, Sara Ghafourian, Sobhan Sekawi, Zamberi Neela, Vasantha Kumari Sadeghifard, Nourkhoda Taherikalani, Morovat Khosravi, Afra Ramli, Ramliza Awang Hamat, Rukman |
author_sort |
Soheili, Sara |
title |
The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
title_short |
The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
title_full |
The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
title_fullStr |
The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
title_full_unstemmed |
The mazef toxin-antitoxin system as an attractive target in clinical isolates of Enterococcus faecium and Enterococcus faecalis |
title_sort |
mazef toxin-antitoxin system as an attractive target in clinical isolates of enterococcus faecium and enterococcus faecalis |
publisher |
Dove Medical Press |
publishDate |
2015 |
url |
http://psasir.upm.edu.my/id/eprint/46648/1/The%20mazef%20toxin-antitoxin%20system%20as%20an%20attractive%20target%20in%20clinical%20isolates%20of%20Enterococcus%20faecium%20and%20Enterococcus%20faecalis.pdf http://psasir.upm.edu.my/id/eprint/46648/ http://www.dovepress.com/drug-design-development-and-therapy-journal |
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1643833806199717888 |
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13.18916 |