Optimization of fish gelatin-alginate-genipin as encapsulating matrices for probiotic application using FCCD-RSM

• Main Authors: Abdul Khalil, Khalilah, Mustafa, Shuhaimi, Mohamad, Rosfarizan, Ariff, Arbakariya, Abdul Manap, Yazid
• Format: Conference or Workshop Item
• Language: English
• Published: IEEE 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/39561/1/Optimization%20of%20fish%20gelatin-alginate-genipin%20as%20encapsulating%20matrices%20for%20probiotic%20application%20using%20FCCD-RSM.pdf; http://psasir.upm.edu.my/id/eprint/39561/

The optimal composition of fish gelatin-alginate-genipin to encapsulate Bifidobacterium pseudocatenulatum G4 was studied. The Face Central Composition Design-Response Surface Methodology (FCCD-RSM) was employed to determine the optimal ration of the matrices in order to obtain high in encapsulation yield (%) and beads strength. Results indicated that optimized matrices could increase the entrapped viable cells and beads strength. The optimal combination of encapsulating matrices was found to be 12.57 % for fish gelatin combined with 5 % alginate and 19.1 mM genipin. Verification experiment confirmed the prediction with low value error and gave indication a good performance prognosis of the optimal formulation.