An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes
A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequen...
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Springer Verlag
1996
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Online Access: | http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf http://psasir.upm.edu.my/id/eprint/39106/ |
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my.upm.eprints.391062015-07-07T08:01:56Z http://psasir.upm.edu.my/id/eprint/39106/ An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes Harikrishna, K. Jampates-Beale, Rachanee Milligan, Stephen B. Gasser, Charles S. A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequence to a previously described stylar chitinase (SK2) isolated from potato. Both proteins lack the diagnostic N-terminal cysteine-rich regions and the C-terminal vacuolar targeting signals of class I chitinases and appear to define a novel type of class II endochitinases associated with flowers. Chi2;1 is expressed predominantly in floral organs and its expression within these organs is temporally regulated. The highest level of expression is found in the transmitting tissue of the style where Chi2;1 mRNA begins to accumulate just prior to anthesis. In vegetative tissue, low levels of Chi2;1 mRNA were detected, and these levels did not increase in response to wounding or treatment with ethephon. mRNA from Chi2;1 orthologs was not detected in most other angiosperms tested, even including some members of the Solanaceae, and it is therefore unlikely that Chi2;1 is essential for stylar function. A fragment containing 1.4 kilobase pairs of 5′-flanking DNA from the Chi2;1 gene was shown to drive high-level expression of an attached reporter gene in the styles of transgenic tomatoes. This fragment has utility for engineering expression of other coding regions in styles. Springer Verlag 1996-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf Harikrishna, K. and Jampates-Beale, Rachanee and Milligan, Stephen B. and Gasser, Charles S. (1996) An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes. Plant Molecular Biology Reporter , 30 (5). pp. 899-911. ISSN 0735-9640; ESSN:1572-9818 10.1007/BF00020802 |
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A gene (pMON9617; Chi2;1) identified by screening a tomato pistil cDNA library has been found to encode a protein similar in sequence to class II chitinases. Using a baculovirus expression system we show that the Chi2;1 protein is an active endochitinase. The Chi2;1 protein is most similar in sequence to a previously described stylar chitinase (SK2) isolated from potato. Both proteins lack the diagnostic N-terminal cysteine-rich regions and the C-terminal vacuolar targeting signals of class I chitinases and appear to define a novel type of class II endochitinases associated with flowers. Chi2;1 is expressed predominantly in floral organs and its expression within these organs is temporally regulated. The highest level of expression is found in the transmitting tissue of the style where Chi2;1 mRNA begins to accumulate just prior to anthesis. In vegetative tissue, low levels of Chi2;1 mRNA were detected, and these levels did not increase in response to wounding or treatment with ethephon. mRNA from Chi2;1 orthologs was not detected in most other angiosperms tested, even including some members of the Solanaceae, and it is therefore unlikely that Chi2;1 is essential for stylar function. A fragment containing 1.4 kilobase pairs of 5′-flanking DNA from the Chi2;1 gene was shown to drive high-level expression of an attached reporter gene in the styles of transgenic tomatoes. This fragment has utility for engineering expression of other coding regions in styles. |
format |
Article |
author |
Harikrishna, K. Jampates-Beale, Rachanee Milligan, Stephen B. Gasser, Charles S. |
spellingShingle |
Harikrishna, K. Jampates-Beale, Rachanee Milligan, Stephen B. Gasser, Charles S. An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
author_facet |
Harikrishna, K. Jampates-Beale, Rachanee Milligan, Stephen B. Gasser, Charles S. |
author_sort |
Harikrishna, K. |
title |
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
title_short |
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
title_full |
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
title_fullStr |
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
title_full_unstemmed |
An endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
title_sort |
endochitinase gene expressed at high levels in the stylar transmitting tissue of tomatoes |
publisher |
Springer Verlag |
publishDate |
1996 |
url |
http://psasir.upm.edu.my/id/eprint/39106/1/An%20endochitinase%20gene%20expressed%20at%20high%20levels.pdf http://psasir.upm.edu.my/id/eprint/39106/ |
_version_ |
1643832327185367040 |
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13.160551 |