Changes of thermostability, organic solvent, and pH stability in Geobacillus zalihae HT1 and its mutant by calcium ion

• Main Authors: Siti Nor Hasmah I,, Masomian, Malihe *, Nor Hafizah AK,, Mohd Shukri MA,, Leow, Thean Chor, Raja Noor Zaliha, R. A. R.
• Format: Article
• Language: English
• Published: MDPI 2019
• Subjects: QR Microbiology
• Online Access: http://eprints.sunway.edu.my/1095/1/Malihe%20Masomian_Changes%20of%20Thermostability.pdf; http://eprints.sunway.edu.my/1095/; http://doi.org/10.3390/ijms20102561

Thermostable T1 lipase from Geobacillus zalihae has been crystallized using counter-diffusion method under space and Earth conditions. The comparison of the three-dimensional structures from both crystallized proteins show differences in the formation of hydrogen bond and ion interactions. Hydrogen...