Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia
This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama. Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase te...
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my.upm.eprints.381042020-04-14T13:58:50Z http://psasir.upm.edu.my/id/eprint/38104/ Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia Zulkhairi Amin, Fatin Aina Sabri, Suriana Ismail, Maznah Chan, Kim Wei Ismail, Norsharina Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Zawawi, Norhasnida This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama. Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health. MDPI 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/38104/1/38104.pdf Zulkhairi Amin, Fatin Aina and Sabri, Suriana and Ismail, Maznah and Chan, Kim Wei and Ismail, Norsharina and Mohd Esa, Norhaizan and Mohd Lila, Mohd Azmi and Zawawi, Norhasnida (2020) Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia. International Journal of Environmental Research and Public Health, 17 (1). art. no. 278. pp. 1-15. ISSN 1661-7827; ESSN: 1660-4601 https://www.mdpi.com/1660-4601/17/1/278 10.3390/ijerph17010278 |
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This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama. Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health. |
format |
Article |
author |
Zulkhairi Amin, Fatin Aina Sabri, Suriana Ismail, Maznah Chan, Kim Wei Ismail, Norsharina Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Zawawi, Norhasnida |
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Zulkhairi Amin, Fatin Aina Sabri, Suriana Ismail, Maznah Chan, Kim Wei Ismail, Norsharina Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Zawawi, Norhasnida Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
author_facet |
Zulkhairi Amin, Fatin Aina Sabri, Suriana Ismail, Maznah Chan, Kim Wei Ismail, Norsharina Mohd Esa, Norhaizan Mohd Lila, Mohd Azmi Zawawi, Norhasnida |
author_sort |
Zulkhairi Amin, Fatin Aina |
title |
Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
title_short |
Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
title_full |
Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
title_fullStr |
Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
title_full_unstemmed |
Probiotic properties of Bacillus strains isolated from stingless bee (Heterotrigona itama) honey collected across Malaysia |
title_sort |
probiotic properties of bacillus strains isolated from stingless bee (heterotrigona itama) honey collected across malaysia |
publisher |
MDPI |
publishDate |
2020 |
url |
http://psasir.upm.edu.my/id/eprint/38104/1/38104.pdf http://psasir.upm.edu.my/id/eprint/38104/ https://www.mdpi.com/1660-4601/17/1/278 |
_version_ |
1665895953915183104 |
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13.201949 |