An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode
A sensitive approach for the determination of Ganoderma boninense DNA is reported based on an electrochemical affinity system using a modified gold sensor. Covalent attachment of probe DNA was achieved by attachment of the amine group to a carboxylic acid group of a 3,3’-dithiodipropionic acid monol...
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Taylor & Francis
2014
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Online Access: | http://psasir.upm.edu.my/id/eprint/36404/1/An%20electrochemical%20biosensor%20for%20the%20determination%20of%20Ganorderma%20boninense%20pathogen%20based%20on%20a%20novel%20modified%20gold%20nanocomposite%20film%20electrode.pdf http://psasir.upm.edu.my/id/eprint/36404/ |
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my.upm.eprints.364042015-10-06T06:56:01Z http://psasir.upm.edu.my/id/eprint/36404/ An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode Dutse, Sabo Wada Yusof, Nor Azah Ahmad, Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh Hajian, Reza A sensitive approach for the determination of Ganoderma boninense DNA is reported based on an electrochemical affinity system using a modified gold sensor. Covalent attachment of probe DNA was achieved by attachment of the amine group to a carboxylic acid group of a 3,3’-dithiodipropionic acid monolayer on a nanocomposite film of gold nanoparticles bound to poly(3,4-ethylenedioxythiophen)–poly(styrenesulfonate) on a gold working electrode. The electrochemical detection of sequence-specific DNA of probe and target DNA hybridization was monitored using a new ruthenium complex [Ru(dppz) 2 (qtpy)Cl 2 ; dppz = dipyrido [3,2–a:2’,3’-c] phenazine; qtpy = 2,2’,-4,4”.4’4”’-quarterpyridyl redox marker. The potential was selected through the study of the electrochemical behavior of trisaminomethane-hydrochloride containing a ethylenediaminetetraacetic acid supporting electrolyte on the bare and modified gold electrode. The effect of the hybridization temperature and time were measured. The sensor demonstrated specific detection for the target over a concentration range of 1.0 × 10− 15 M to 1.0 × 10− 9 M with a detection limit of 1.59 × 10− 17 M. Control experiments verified the specificity of the biosensor in the presence of a single mismatched DNA sequence. This detection technology was shown to be effective in terms of sensitivity and selectivity of hybridization events and is a promising device for early detection of Ganoderma boninense and other pathogenic threat agents. Taylor & Francis 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/36404/1/An%20electrochemical%20biosensor%20for%20the%20determination%20of%20Ganorderma%20boninense%20pathogen%20based%20on%20a%20novel%20modified%20gold%20nanocomposite%20film%20electrode.pdf Dutse, Sabo Wada and Yusof, Nor Azah and Ahmad, Haslina and Hussein, Mohd Zobir and Zainal, Zulkarnain and Hushiarian, Roozbeh and Hajian, Reza (2014) An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode. Analytical Letters, 47 (5). pp. 819-832. ISSN 0003-2719; ESSN: 1532-236X 10.1080/00032719.2013.858261 |
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A sensitive approach for the determination of Ganoderma boninense DNA is reported based on an electrochemical affinity system using a modified gold sensor. Covalent attachment of probe DNA was achieved by attachment of the amine group to a carboxylic acid group of a 3,3’-dithiodipropionic acid monolayer on a nanocomposite film of gold nanoparticles bound to poly(3,4-ethylenedioxythiophen)–poly(styrenesulfonate) on a gold working electrode. The electrochemical detection of sequence-specific DNA of probe and target DNA hybridization was monitored using a new ruthenium complex [Ru(dppz) 2 (qtpy)Cl 2 ; dppz = dipyrido [3,2–a:2’,3’-c] phenazine; qtpy = 2,2’,-4,4”.4’4”’-quarterpyridyl redox marker. The potential was selected through the study of the electrochemical behavior of trisaminomethane-hydrochloride containing a ethylenediaminetetraacetic acid supporting electrolyte on the bare and modified gold electrode. The effect of the hybridization temperature and time were measured. The sensor demonstrated specific detection for the target over a concentration range of 1.0 × 10− 15 M to 1.0 × 10− 9 M with a detection limit of 1.59 × 10− 17 M. Control experiments verified the specificity of the biosensor in the presence of a single mismatched DNA sequence. This detection technology was shown to be effective in terms of sensitivity and selectivity of hybridization events and is a promising device for early detection of Ganoderma boninense and other pathogenic threat agents. |
format |
Article |
author |
Dutse, Sabo Wada Yusof, Nor Azah Ahmad, Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh Hajian, Reza |
spellingShingle |
Dutse, Sabo Wada Yusof, Nor Azah Ahmad, Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh Hajian, Reza An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
author_facet |
Dutse, Sabo Wada Yusof, Nor Azah Ahmad, Haslina Hussein, Mohd Zobir Zainal, Zulkarnain Hushiarian, Roozbeh Hajian, Reza |
author_sort |
Dutse, Sabo Wada |
title |
An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
title_short |
An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
title_full |
An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
title_fullStr |
An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
title_full_unstemmed |
An electrochemical biosensor for the determination of Ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
title_sort |
electrochemical biosensor for the determination of ganorderma boninense pathogen based on a novel modified gold nanocomposite film electrode |
publisher |
Taylor & Francis |
publishDate |
2014 |
url |
http://psasir.upm.edu.my/id/eprint/36404/1/An%20electrochemical%20biosensor%20for%20the%20determination%20of%20Ganorderma%20boninense%20pathogen%20based%20on%20a%20novel%20modified%20gold%20nanocomposite%20film%20electrode.pdf http://psasir.upm.edu.my/id/eprint/36404/ |
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1643831730678792192 |
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13.18916 |