Method of in vitro isolation, proliferation and differenttiation of mesenchymal stem cells into osteoblasts in healthy and pathologic conditions

• Main Authors: Ibrahim, Rashid, Kamran, Kareh, Abu Bakar @ Zakaria, Md Zuki, Tengku Ibrahim, Tengku Azmi
• Format: Patent
• Published: 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/32141/

The present invention provides a method of in vitro cultivation of mesenchymal stem cells into osteoblasts, in which total bone marrow taken from a mammal is cultivated in a complete medium containing an increased amount of fetal bovine serum under standard conditions, subsequently the non-adherent...