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Method for the purification of interferon.

The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and...

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Main Authors: Ling, Tau Chuan, Lin, Yu Kiat, Ooi, Chien Wei, Ariff, Arbakariya
Format: Patent
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/32048/
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spelling my.upm.eprints.320482014-09-04T04:38:40Z http://psasir.upm.edu.my/id/eprint/32048/ Method for the purification of interferon. Ling, Tau Chuan Lin, Yu Kiat Ooi, Chien Wei Ariff, Arbakariya The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems. 2011-01-12 Patent NonPeerReviewed Ling Tau Chuan (2011) Method for the purification of interferon. PI2011000150.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present invention discloses a method for the purification of a recombinant microbial Interferon α-2b in an aqueous two-phase system comprising a combination polymer and salt solution. In an embodiment of the present invention, the aqueous two-phase system comprising a combination of polymer and salt solution includes polyethylene glycol (PEG)-potassium phosphate, polyethylene glycol (PEG)-ammonium sulfate, polyethylene glycol (PEG)-sodium carbonate, polyethylene glycol (PEG)-sodium citrate and polyethylene glycol (PEG)-magnesium sulfate. The preferred aqueous two-phase system is the polyethylene glycol (PEG)-potassium phosphate system due to the benefits of low cost and short phase separation time as compared to other systems.
format Patent
author Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
spellingShingle Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
Method for the purification of interferon.
author_facet Ling, Tau Chuan
Lin, Yu Kiat
Ooi, Chien Wei
Ariff, Arbakariya
author_sort Ling, Tau Chuan
title Method for the purification of interferon.
title_short Method for the purification of interferon.
title_full Method for the purification of interferon.
title_fullStr Method for the purification of interferon.
title_full_unstemmed Method for the purification of interferon.
title_sort method for the purification of interferon.
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/32048/
_version_ 1643830494742183936
score 13.160551

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