Purification and characterization of membrane-bound polyphenoloxidase (mPPO) from Snake fruit [Salacca zalacca (Gaertn.) Voss].

• Main Authors: Mohd Zaini, Nurul Aqilah, Osman, Azizah, Abdul Hamid, Azizah, Ebrahimpour, Afshin, Saari, Nazamid
• Format: Article
• Language: English; English
• Published: Elsevier 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/30443/1/Purification%20and%20characterization%20of%20membrane.pdf; http://psasir.upm.edu.my/id/eprint/30443/

Membrane-bound polyphenoloxidase (mPPO) an oxidative enzyme which is responsible for the undesirable browning reaction in Snake fruit (Salacca zalacca (Gaertn.) Voss) was investigated. The enzyme was extracted using a non-ionic detergent (Triton X-114), followed by temperature-induced phase partitio...