Downregulation of RhoGDIα increased migration and invasion of ER+ MCF7 and ER− MDA-MB-231 breast cancer cells.
Rho GDp dissociation inhibitors (RhoGDIs) can inhibit cell motility, invasion, and metastasis in cancer by inactivating the RhoGTpases. A member of RhoGDI family has been consistently shown to interact with estrogen receptor (eR), and change its transcriptional activity. eR is a receptor known t...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English English |
Published: |
Landes Bioscience
2013
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Online Access: | http://psasir.upm.edu.my/id/eprint/28133/1/Downregulation%20of%20RhoGDI%CE%B1%20increased%20migration%20and%20invasion%20of%20ER.pdf http://psasir.upm.edu.my/id/eprint/28133/ http://www.landesbioscience.com/journals/celladhesion/toc/volume/7/issue/3/ |
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Summary: | Rho GDp dissociation inhibitors (RhoGDIs) can inhibit cell motility, invasion, and metastasis in cancer by inactivating
the RhoGTpases. A member of RhoGDI family has been consistently shown to interact with estrogen receptor (eR), and
change its transcriptional activity. eR is a receptor known to be inversely correlated with cell motility and invasion in
breast cancer. The consequence of RhoGDIα activity on migration and invasion of eR+ and eR− breast cancers is not clear.
The aim of our study was to investigate the possible opposing effect of RhoGDIα on the migration and invasion of eR+
MCF7 and eR− MDA-MB-231 breast cancer cells. RhoGDIα was downregulated using short interfering RNA (siRNA) and
upregulated using GFp-tagged ORF clone of RhoGDIα, and their ability for migration and invasion was assayed using
transwell chambers. It was found that the silencing of RhoGDIα in MCF7 and MDA-MB-231 cells significantly increased
migration and invasion of these cells into the lower surface of porous membrane of the chambers. Overexpression of
RhoGDIα in MCF7 cells suppressed their migration and invasion, but no significant effect was found on MDA-MB-231
cells. Our results indicate that the downregulation of RhoGDIα similarly affects the in vitro migration and invasion of eR+
MCF7 and eR− MDA-MB-231 cells. however, our assays are differently affected by the upregulation of RhoGDIα in these
two cell lines and this may be due to the differences in eR expression, primary invasive ability and/or other molecules
between these two cell line models which warrant further investigation. |
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