Effects of medium and culture conditions on folate production by Streptococcus thermophilus BAA-250.

• Main Authors: Mousavi, Seyed Sadegh, Moeini, Hassan, Mohamad, Rosfarizan, Dinarvand, Mojdeh, Ariff, Arbakariya, Foo, Hooi Ling, Abdul Rahim, Raha
• Format: Article
• Language: English; English
• Published: 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/28123/1/Effects%20of%20medium%20and%20culture%20conditions%20on%20folate%20production%20by%20Streptococcus%20thermophilus%20BAA.pdf; http://psasir.upm.edu.my/id/eprint/28123/; http://researchinbiotechnology.com/issue/view/21

The present study was conducted to investigate the effects of culture conditions on folate production by Streptococcus thermophilus BAA-250. Lactose (3g/L) and yeast extract (20g/L) were found to be the more suitable carbon and nitrogen sources for folate production by S. thermophilus BAA-250. para-aminobenzoic acid (pABA) higher than 1 µM had no significant effect on folate biosynthesis. The optimum pH for folate production was shown to be 7.0 with a folate yield and productivity of 54.53 (µg/L) and 2.27 (µg/L.h), respectively. Optimum folate production obtained in the presence of lactose and yeast extract in a controlled pH of 7 during batch fermentation in bioreactor. Kinetic studies indicated that folate production by S. thermophilus is growth-associated process.