Dynamics of the bacterially expressed conserved immunogenic region of the human respiratory syncytial virus G protein.

• Main Authors: Jalilian, Farid Azizi, Jahanshiri, Fatemeh, Sekawi, Zamberi, Omar, Abdul Rahman, Yusoff, Khatijah
• Format: Article
• Language: English; English
• Published: Wiley-Blackwell 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/25358/1/Dynamics%20of%20the%20bacterially%20expressed%20conserved%20immunogenic%20region%20of%20the%20human%20respiratory%20syncytial%20virus%20G%20protein.pdf; http://psasir.upm.edu.my/id/eprint/25358/; http://onlinelibrary.wiley.com/

Despite all efforts, there is still no effective vaccine available against the human respiratory syncytial virus (HRSV) that is a major cause of severe lower respiratory tract disease in infants and the elderly. In this review, we examined the potential of the conserved immunogenic region (residues 122-226) of the HRSV glycoprotein G alone as the inducer of neutralizing antibodies against this virus. The Escherichia coli produced recombinant conserved region of G (designated as G domain), which was used for rabbit immunization. Although rabbit is a semipermissive host for HRSV, our result showed that the polyclonal antibodies against the G domain protein could strongly neutralize the virus (69.3%), suggesting that the G immunogenic region of HRSV alone has a great potential in vaccine development. To our knowledge, this is the first report in which neutralizing antibodies to respiratory syncytial virus have been evoked using bacterially expressed G immunogenic domain protein without any adjuvant.