Detection of eight common β-globin gene mutation in thalassemia major patients using real time polymerase chain reaction (PCR)-high resolution melting and EvaGreen™ dye

• Main Authors: Marash, Seyed Jalal, Eshkoor, Sima Ataollahi, Mirinargesi, Mir Saed, Sarookhani, Mohammad Reza, Rahmat, Asmah, Ismail, Patimah
• Format: Article
• Language: English
• Published: Academic Journals 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/24710/1/24710.pdf; http://psasir.upm.edu.my/id/eprint/24710/; http://www.academicjournals.org/journal/AJB/article-abstract/5D9B3BC30381

The aim of the present study was to determine the phenotype and genotype frequency of the most common β-globin mutations among the thalassemia major (TM) patients, using high-resolution melting (HRM) curve analysis as a novel, reliable and rapid scanning method. In this descriptive-analytical study,...