Detection of Bcl-2 gene in leukaemic rats using an EvaGreen real-time RT-PCT assay

• Main Authors: Haron, Nursyuhada, Hamzah, Hazilawati, Al-Salih, Hutheyfa A. H., Shaari, Mohd Rosly, Sithambaram, Shanmugavelu, Mohamed Mustapha, Noordin, Sabri, Jasni
• Format: Article
• Language: English
• Published: Universiti Putra Malaysia Press 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/25343/1/24%20Pg%20373-380.pdf; http://psasir.upm.edu.my/id/eprint/25343/; http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2034%20(2)%20Aug.%202011/24%20Pg%20373-380.pdf

Bcl-2 is an anti-apoptotic gene that is involved in the apoptosis process. Suppression of apoptosis by anti- apoptotic gene can contribute to the occurrence of diseases such as leukaemia. The objectives of this study were 2-folds: first, to compare the sensitivity of an EvaGreen quantitative real-t...