Bixa orellana leaves extract inhibits bradykinin-induced inflammation through suppression of nitric oxide production

• Main Authors: Yong, Yoke Keong, Abdul Kadir, Arifah, Sukardi, Sabrina, Abd Hamid @ Abdul Razak, Roslida, Somchit, Muhammad Nazrul, Ahmad, Zuraini
• Format: Article
• Language: English
• Published: Karger 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/24585/1/Bixa%20orellana%20leaves%20extract%20inhibits%20bradykinin.pdf; http://psasir.upm.edu.my/id/eprint/24585/; http://www.karger.com/Article/Abstract/319907

Objective: The present study was conducted to assess the anti-inflammatory effect of a crude aqueous extract of Bixa orellana leaves (AEBO) and to examine the possible involvement of nitric oxide (NO) in its anti-inflammatory mechanism. Materials and Methods: The air-dried, powdered leaves were soaked in distilled water (1:20 w/v) at 50°C for 24 h and the supernatant obtained was freeze-dried (yield 8.5% w/w). The dosage was recorded as the mass of extract per kg b.w. of rats in all inflammatory assays (bradykinin-induced paw edema, peritoneal vascular permeability and NO assay). Results: Pretreatment with AEBO for 4 consecutive days exhibited significant inhibitory activity against inflammatory models, the bradykinin-induced hind paw edema model and bradykinin-induced increased peritoneal vascular permeability at both doses in dose-dependent manner. In addition, AEBO was also found to significantly suppress the production of NO at doses of 50 and 150 mg/kg. Conclusion: This study provides scientific data to support the traditional use of B. orellana leaves in treating inflammation. Results from this study suggest that AEBO exerts anti-inflammatory effects. Part of this anti-inflammatory effect may be associated with its antibradykinin activity and may be related to a reduction of the NO production.