Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli
Several medium formulations were screened for the production of a thermostable and organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase production (28.9 ± 4.1 IU/mL) was obtained in Luria Bertani medium with the addition of 1% (w/v) glucose. The medium formulati...
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my.upm.eprints.224102016-09-27T02:38:33Z http://psasir.upm.edu.my/id/eprint/22410/ Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli Nelofer, Rubina Ramanan, Ramakrishnan Nagasundara Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya Several medium formulations were screened for the production of a thermostable and organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase production (28.9 ± 4.1 IU/mL) was obtained in Luria Bertani medium with the addition of 1% (w/v) glucose. The medium formulation and fermentation conditions were then subjected to sequential optimization. Using a Plackett-Burman design, glucose, NaCl, temperature and induction time were found to be the most significant variables affecting lipase production, and these were then optimized using response surface methodology (RSM). The large value of R 2 (0.979) showed that the quadratic model used for the prediction is highly significant. The optimum levels of these four significant variables (glucose, NaCl, temperature and induction time) as predicted by RSM were 32.4 g/L, 5 g/L, 31.7°C and 2.1 h, respectively. The amount of lipase activity (50.2 ± 4.5 IU/mL) produced under these optimal conditions fitted well to the value (48.9 IU/mL) predicted by RSM. Production of lipase in optimized fermentation was about 2.5-fold higher than in non-optimized fermentation. Springer 2011-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22410/1/Sequential%20optimization%20of%20production%20of%20a%20thermostable%20and%20organic%20solvent%20tolerant%20lipase%20by%20recombinant%20Escherichia%20coli.pdf Nelofer, Rubina and Ramanan, Ramakrishnan Nagasundara and Raja Abdul Rahman, Raja Noor Zaliha and Basri, Mahiran and Ariff, Arbakariya (2011) Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli. Annals of Microbiology, 61 (3). pp. 535-544. ISSN 1590-4261; ESSN: 1869-2044 http://link.springer.com/article/10.1007/s13213-010-0170-9 10.1007/s13213-010-0170-9 |
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Several medium formulations were screened for the production of a thermostable and organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase production (28.9 ± 4.1 IU/mL) was obtained in Luria Bertani medium with the addition of 1% (w/v) glucose. The medium formulation and fermentation conditions were then subjected to sequential optimization. Using a Plackett-Burman design, glucose, NaCl, temperature and induction time were found to be the most significant variables affecting lipase production, and these were then optimized using response surface methodology (RSM). The large value of R 2 (0.979) showed that the quadratic model used for the prediction is highly significant. The optimum levels of these four significant variables (glucose, NaCl, temperature and induction time) as predicted by RSM were 32.4 g/L, 5 g/L, 31.7°C and 2.1 h, respectively. The amount of lipase activity (50.2 ± 4.5 IU/mL) produced under these optimal conditions fitted well to the value (48.9 IU/mL) predicted by RSM. Production of lipase in optimized fermentation was about 2.5-fold higher than in non-optimized fermentation. |
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Article |
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Nelofer, Rubina Ramanan, Ramakrishnan Nagasundara Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya |
spellingShingle |
Nelofer, Rubina Ramanan, Ramakrishnan Nagasundara Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
author_facet |
Nelofer, Rubina Ramanan, Ramakrishnan Nagasundara Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Ariff, Arbakariya |
author_sort |
Nelofer, Rubina |
title |
Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
title_short |
Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
title_full |
Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
title_fullStr |
Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
title_full_unstemmed |
Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli |
title_sort |
sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant escherichia coli |
publisher |
Springer |
publishDate |
2011 |
url |
http://psasir.upm.edu.my/id/eprint/22410/1/Sequential%20optimization%20of%20production%20of%20a%20thermostable%20and%20organic%20solvent%20tolerant%20lipase%20by%20recombinant%20Escherichia%20coli.pdf http://psasir.upm.edu.my/id/eprint/22410/ http://link.springer.com/article/10.1007/s13213-010-0170-9 |
_version_ |
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13.188404 |