Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods

• Main Authors: Lee, Hai Yen, Chai, Lay Ching, Pui, Chai Fung, Robin, Tunung, Wong, Woan Chwen, Mustafa, Shuhaimi, Cheah, Yoke Kqueen, Mohamad Ghazali, Farinazleen, Nishibuchi, Mitsuaki, Radu, Son
• Format: Article
• Language: English; English
• Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2011
• Online Access: http://psasir.upm.edu.my/id/eprint/22236/1/22236.pdf; http://psasir.upm.edu.my/id/eprint/22236/; http://www.ifrj.upm.edu.my/volume-18-2011.html

In this study, Listeria spp. were isolated from naturally contaminated samples of beef burger, minced beef and sliced cheese using PALCAM and CHROMagar™. Samples were enriched with FDA-BAM method and plated on PALCAM and CHROMagar™ Listeria before confirmation using PCR on hlyA and LLO toxin genes...