Method for producing a recombinant thermostable geobacillus T1 lipase

The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography pu...

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Main Authors: Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar, Basri, Mahiran, Leow, Thean Chor
Format: Patent
Published: 2008
Online Access:http://psasir.upm.edu.my/id/eprint/20390/
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spelling my.upm.eprints.203902016-09-27T02:09:07Z http://psasir.upm.edu.my/id/eprint/20390/ Method for producing a recombinant thermostable geobacillus T1 lipase Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. 2008-11-19 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2008) Method for producing a recombinant thermostable geobacillus T1 lipase. UNSPECIFIED.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein.
format Patent
author Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
Leow, Thean Chor
spellingShingle Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
Leow, Thean Chor
Method for producing a recombinant thermostable geobacillus T1 lipase
author_facet Raja Abdul Rahman, Raja Noor Zaliha
Salleh, Abu Bakar
Basri, Mahiran
Leow, Thean Chor
author_sort Raja Abdul Rahman, Raja Noor Zaliha
title Method for producing a recombinant thermostable geobacillus T1 lipase
title_short Method for producing a recombinant thermostable geobacillus T1 lipase
title_full Method for producing a recombinant thermostable geobacillus T1 lipase
title_fullStr Method for producing a recombinant thermostable geobacillus T1 lipase
title_full_unstemmed Method for producing a recombinant thermostable geobacillus T1 lipase
title_sort method for producing a recombinant thermostable geobacillus t1 lipase
publishDate 2008
url http://psasir.upm.edu.my/id/eprint/20390/
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score 13.209306