Method for producing a recombinant thermostable geobacillus T1 lipase
The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography pu...
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2008
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my.upm.eprints.203902016-09-27T02:09:07Z http://psasir.upm.edu.my/id/eprint/20390/ Method for producing a recombinant thermostable geobacillus T1 lipase Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. 2008-11-19 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2008) Method for producing a recombinant thermostable geobacillus T1 lipase. UNSPECIFIED. |
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description |
The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. |
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Patent |
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Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor |
spellingShingle |
Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor Method for producing a recombinant thermostable geobacillus T1 lipase |
author_facet |
Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor |
author_sort |
Raja Abdul Rahman, Raja Noor Zaliha |
title |
Method for producing a recombinant thermostable geobacillus T1 lipase |
title_short |
Method for producing a recombinant thermostable geobacillus T1 lipase |
title_full |
Method for producing a recombinant thermostable geobacillus T1 lipase |
title_fullStr |
Method for producing a recombinant thermostable geobacillus T1 lipase |
title_full_unstemmed |
Method for producing a recombinant thermostable geobacillus T1 lipase |
title_sort |
method for producing a recombinant thermostable geobacillus t1 lipase |
publishDate |
2008 |
url |
http://psasir.upm.edu.my/id/eprint/20390/ |
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1643827301310267392 |
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13.209306 |