Method for producing a recombinant thermostable geobacillus T1 lipase
The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography pu...
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| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/20390/ |
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| Summary: | The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. |
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