Selection of high affinity ligands to hepatitis B core antigen from a phage-displayed cyclic peptide library.

• Main Authors: Ho, Kok Lian, Yusoff, Khatijah, Seow, Heng Fong, Tan, Wen Siang
• Format: Article
• Language: English; English
• Published: Wiley-Blackwell 2003
• Online Access: http://psasir.upm.edu.my/id/eprint/16650/1/Selection%20of%20high%20affinity%20ligands%20to%20hepatitis%20B%20core%20antigen%20from%20a%20phage.pdf; http://psasir.upm.edu.my/id/eprint/16650/; http://as.wiley.com/WileyCDA/Brand/id-35.html

M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that...