Cover Image

PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.

The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N...

Full description

Saved in:
Bibliographic Details
Main Authors: Shafee, Norazizah, Kaluz, Stefan, Ru, Ning, Stanbridge, Eric John
Format: Article
Language:English
Published: Elsevier 2009
Online Access:http://psasir.upm.edu.my/id/eprint/16221/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.16221
record_format eprints
spelling my.upm.eprints.162212014-02-11T04:41:11Z http://psasir.upm.edu.my/id/eprint/16221/ PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. Shafee, Norazizah Kaluz, Stefan Ru, Ning Stanbridge, Eric John The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N-ras allele), correlated with increased PI3K activity. Constitutive activation of the PI3K pathway in MCH603/PI3Kact cells increased HIF-1α but, surprisingly, decreased CAIX levels. The cell-type specific inhibitory effect on CAIX was confirmed at the transcriptional level whereas epigenetic modifications of CA9 were ruled out. In summary, our data do not substantiate the generalization that PI3K upregulation leads to increased HIF activity. Elsevier 2009-09-08 Article PeerReviewed Shafee, Norazizah and Kaluz, Stefan and Ru, Ning and Stanbridge, Eric John (2009) PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. Cancer Letters, 282 (1). pp. 109-115. ISSN 0304-3835; ESSN: 1872-7980 10.1016/j.canlet.2009.03.004 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N-ras allele), correlated with increased PI3K activity. Constitutive activation of the PI3K pathway in MCH603/PI3Kact cells increased HIF-1α but, surprisingly, decreased CAIX levels. The cell-type specific inhibitory effect on CAIX was confirmed at the transcriptional level whereas epigenetic modifications of CA9 were ruled out. In summary, our data do not substantiate the generalization that PI3K upregulation leads to increased HIF activity.
format Article
author Shafee, Norazizah
Kaluz, Stefan
Ru, Ning
Stanbridge, Eric John
spellingShingle Shafee, Norazizah
Kaluz, Stefan
Ru, Ning
Stanbridge, Eric John
PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
author_facet Shafee, Norazizah
Kaluz, Stefan
Ru, Ning
Stanbridge, Eric John
author_sort Shafee, Norazizah
title PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
title_short PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
title_full PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
title_fullStr PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
title_full_unstemmed PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
title_sort pi3k/akt activity has variable cell-specific effects on expression of hif target genes, caix and vegf, in human cancer cell lines.
publisher Elsevier
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/16221/
_version_ 1643826149463162880
score 13.211869

Similar Items