Molecular characterisation of phaCAB from Comamonas sp. EB172 for functional expression in Escherichia coli JM109

• Main Authors: Yee, Lian Ngit, Chuah, Jo Ann, Chong, Mei Ling, Phang, Lai Yee, Abdul Rahim, Raha, Sudesh, Kumar, Hassan, Mohd Ali
• Format: Article
• Language: English
• Published: Elsevier 2012
• Online Access: http://psasir.upm.edu.my/id/eprint/16169/1/Molecular%20characterisation%20of%20phaCAB%20from%20Comamonas%20sp.%20EB172%20for%20functional%20expression%20in%20Escherichia%20coli%20JM109.pdf; http://psasir.upm.edu.my/id/eprint/16169/; http://www.sciencedirect.com/science/article/pii/S0944501312000043

In this study, PHA biosynthesis operon of Comamonas sp. EB172, an acid-tolerant strain, consisting of three genes encoding acetyl-CoA acetyltransferase (phaACo gene, 1182 bp), acetoacetyl-CoA reductase (phaBCo gene, 738 bp) and PHA synthase, class I (phaCCo gene, 1694 bp) were identified. Sequence a...