Optimization of an induction strategy for improving interferon-α2b production in the periplasm of Escherichia coli using response surface methodology

• Main Authors: Azaman, Siti Nor Ani, Ramakrishnan, Nagasundara Ramanan, Tan, Joo Shun, Abdul Rahim, Raha, Abdullah, Mohd Puad, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Portland Press 2010
• Online Access: http://psasir.upm.edu.my/id/eprint/15962/1/Optimization%20of%20an%20induction%20strategy%20for%20improving%20interferon.pdf; http://psasir.upm.edu.my/id/eprint/15962/

Induction strategies for the periplasmic production of recombinant human IFN-alpha2b (interferon-alpha2b) by recombinant Escherichia coli Rosetta-gami 2(DE3) were optimized in shake-flask cultures using response surface methodology based on the central composite design. The factors included in the p...