Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells

BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic...

Full description

Saved in:
Bibliographic Details
Main Authors: Chong, Sun Li, Mou, Duen Gang, Lim, Saw Hoon, Ali, Abdul Manaf, Tey, Beng Ti
Format: Article
Language:English
Published: Society of Chemical Industry 2009
Online Access:http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf
http://psasir.upm.edu.my/id/eprint/14517/
https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.2228
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.14517
record_format eprints
spelling my.upm.eprints.145172019-04-08T08:51:07Z http://psasir.upm.edu.my/id/eprint/14517/ Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells Chong, Sun Li Mou, Duen Gang Lim, Saw Hoon Ali, Abdul Manaf Tey, Beng Ti BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic conditions (32 °C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30%). RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30% FBS supplemented, 32 °C) were 1.38‐ and 1.34‐fold greater than the control culture (10% FBS supplemented, 37 °C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth. CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells. Society of Chemical Industry 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf Chong, Sun Li and Mou, Duen Gang and Lim, Saw Hoon and Ali, Abdul Manaf and Tey, Beng Ti (2009) Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells. Journal of Chemical Technology and Biotechnology, 84 (11). pp. 1674-1680. ISSN 0268-2575; ESSN: 1097-4660 https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.2228 10.1002/jctb.2228
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic conditions (32 °C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30%). RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30% FBS supplemented, 32 °C) were 1.38‐ and 1.34‐fold greater than the control culture (10% FBS supplemented, 37 °C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth. CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells.
format Article
author Chong, Sun Li
Mou, Duen Gang
Lim, Saw Hoon
Ali, Abdul Manaf
Tey, Beng Ti
spellingShingle Chong, Sun Li
Mou, Duen Gang
Lim, Saw Hoon
Ali, Abdul Manaf
Tey, Beng Ti
Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
author_facet Chong, Sun Li
Mou, Duen Gang
Lim, Saw Hoon
Ali, Abdul Manaf
Tey, Beng Ti
author_sort Chong, Sun Li
title Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
title_short Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
title_full Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
title_fullStr Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
title_full_unstemmed Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
title_sort enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells
publisher Society of Chemical Industry
publishDate 2009
url http://psasir.upm.edu.my/id/eprint/14517/1/Enhancement%20of%20monoclonal%20antibody%20productivity%20by%20promoting%20active%20hypothermic%20growth%20in%20hybridoma%20cells.pdf
http://psasir.upm.edu.my/id/eprint/14517/
https://onlinelibrary.wiley.com/doi/abs/10.1002/jctb.2228
_version_ 1643825655960305664
score 13.209306