Enhanced interferon-α2b production in periplasmic space of Escherichia coli through medium optimization using response surface method

• Main Authors: Tan, Joo Shun, Ramanan, Ramakrishnan Nagasundara, Azaman, Siti Nor Ani, Ling, Tau Chuan, Mustafa, Shuhaimi, Ariff, Arbakariya
• Format: Article
• Language: English
• Published: Bentham Open 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/14509/1/Enhanced%20interferon-%CE%B12b%20production%20in%20periplasmic%20space%20of%20Escherichia%20coli%20through%20medium%20optimization%20using%20response%20surface%20method.pdf; http://psasir.upm.edu.my/id/eprint/14509/; https://www.benthamopen.com/ABSTRACT/TOBIOTJ-3-117

The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components t...