Engineering of E. coli for increased production of L-lactic acid

• Main Authors: Tengku Zainal Mulok, Tengku Elida, Chong, Mei Ling, Shirai, Yoshihito, Abdul Rahim, Raha, Hassan, Mohd Ali
• Format: Article
• Language: English
• Published: Academic Journals 2009
• Online Access: http://psasir.upm.edu.my/id/eprint/14503/1/14503.pdf; http://psasir.upm.edu.my/id/eprint/14503/; http://www.academicjournals.org/journal/AJB/article-abstract/EACCE0F9284

An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and...