Engineering of E. coli for increased production of L-lactic acid
An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and...
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Academic Journals
2009
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Online Access: | http://psasir.upm.edu.my/id/eprint/14503/1/14503.pdf http://psasir.upm.edu.my/id/eprint/14503/ http://www.academicjournals.org/journal/AJB/article-abstract/EACCE0F9284 |
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my.upm.eprints.145032016-05-03T11:13:48Z http://psasir.upm.edu.my/id/eprint/14503/ Engineering of E. coli for increased production of L-lactic acid Tengku Zainal Mulok, Tengku Elida Chong, Mei Ling Shirai, Yoshihito Abdul Rahim, Raha Hassan, Mohd Ali An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and western blotting method confirmed the presence of recombinant L-LDH enzyme with the approximate size of 40kD. The activity of L-lactate dehydrogenase was achieved at 170 U ml¯¹. E.coli BAD85 was found to produce 0.62 g l¯¹ of lactic acid from 1 g 1¯¹ of fructose in 24 h. L-ldh gene from was successfully transformed into E.coli SZ85 with the maximum production of L-lactic acid at 0.62 g l¯¹. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/14503/1/14503.pdf Tengku Zainal Mulok, Tengku Elida and Chong, Mei Ling and Shirai, Yoshihito and Abdul Rahim, Raha and Hassan, Mohd Ali (2009) Engineering of E. coli for increased production of L-lactic acid. African Journal of Biotechnology, 8 (18). art. no. EACCE0F9284. pp. 4597-4603. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/EACCE0F9284 |
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An over-expressed L-ldh gene derivative of Escherichia coli BAD-ldh was developed. L-ldh gene from Enterococcus facelis KK1 consisted of an open reading frame of 954 bp encoding 316 amino acids. L-ldh gene was cloned into pBAD vector and transformed into E.coli SZ85 by electroporation. SDS-page and western blotting method confirmed the presence of recombinant L-LDH enzyme with the approximate size of 40kD. The activity of L-lactate dehydrogenase was achieved at 170 U ml¯¹. E.coli BAD85 was found to produce 0.62 g l¯¹ of lactic acid from 1 g 1¯¹ of fructose in 24 h. L-ldh gene from was successfully transformed into E.coli SZ85 with the maximum production of L-lactic acid at 0.62 g l¯¹. |
format |
Article |
author |
Tengku Zainal Mulok, Tengku Elida Chong, Mei Ling Shirai, Yoshihito Abdul Rahim, Raha Hassan, Mohd Ali |
spellingShingle |
Tengku Zainal Mulok, Tengku Elida Chong, Mei Ling Shirai, Yoshihito Abdul Rahim, Raha Hassan, Mohd Ali Engineering of E. coli for increased production of L-lactic acid |
author_facet |
Tengku Zainal Mulok, Tengku Elida Chong, Mei Ling Shirai, Yoshihito Abdul Rahim, Raha Hassan, Mohd Ali |
author_sort |
Tengku Zainal Mulok, Tengku Elida |
title |
Engineering of E. coli for increased production of L-lactic acid |
title_short |
Engineering of E. coli for increased production of L-lactic acid |
title_full |
Engineering of E. coli for increased production of L-lactic acid |
title_fullStr |
Engineering of E. coli for increased production of L-lactic acid |
title_full_unstemmed |
Engineering of E. coli for increased production of L-lactic acid |
title_sort |
engineering of e. coli for increased production of l-lactic acid |
publisher |
Academic Journals |
publishDate |
2009 |
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http://psasir.upm.edu.my/id/eprint/14503/1/14503.pdf http://psasir.upm.edu.my/id/eprint/14503/ http://www.academicjournals.org/journal/AJB/article-abstract/EACCE0F9284 |
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