Calcium-induced stabilization of α-amylase against guanidine hydrochloride denaturation
Guanidine hydrochloride (GdnHCl) denaturation of native and Ca-depleted Bacillus licheniformis α-amylase (BLA) was investigated both in the absence and presence of 2 mM calcium chloride (CaCl2)using circular dichroism, fluorescence spectroscopy and biological activity. In both states (Cadepleted and...
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Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Academic Journals
2010
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Online Access: | http://psasir.upm.edu.my/id/eprint/13474/1/13474.pdf http://psasir.upm.edu.my/id/eprint/13474/ http://www.academicjournals.org/journal/AJB/article-abstract/9903FE419346 |
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