Amelioration of mangosteen micro propagation through leaf and seed segments (Garcinia Mangostana L.)

• Main Authors: Sirchi, Mohammad Hossein Torabi, Abdul Kadir, Mihdzar, Abdul Aziz, Maheran, Abdul Rashid, Azmi, Rafat, Arash, Javadi, Mohammad Bagher
• Format: Article
• Language: English
• Published: Academic Journals 2008
• Online Access: http://psasir.upm.edu.my/id/eprint/12999/1/12999.pdf; http://psasir.upm.edu.my/id/eprint/12999/; http://www.academicjournals.org/journal/AJB/article-abstract/5BED1AE7507

A micro-propagation system was developed for mangosteen (Garcinia Mangostana L.) using leaf sections and mature seeds. This study investigated the effect of various modified Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurin (BAP) alone or in combination with α-naphthalene acetic acid (NAA) on multiple shoot induction from leaf and half seed explants. Among the treatments, leaf explants on medium supplemented with 2.0 mgL־¹ (w/v) of BAP gave the highest percentage of shoot formation (70.07%), highest number of shoots formed per explant (46.5%) and showed high mean shoot height (0.48 cm). It was observed that medium with 0.5 mgL־¹ NAA, did not induce shoot organogenesis, but induced callus in 90% of the leaf explants. Seed explantsgave the highest percentage of shoot formation (60.2%) and mean number of shoots per explant (9.5) on medium supplemented with 20 mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA. However, highest mean shoot height (0.69 cm) was observed on medium supplemented with 10 mgL־¹ (w/v) BAP and 10 mgL־¹ (w/v) NAA. Callus induction from seeds was also observed in most of the treatments. Medium-supplemented 20 mgL־¹ (w/v) NAA gave the highest percentage of callus formation (83.36%). Subsequently, roots were induced from the shoots by pretreating these shoots in various indol-3-butyric acid (IBA) and NAA concentrations in various MS salt strengths. Root formation was obtained on one-quarter strength MS salt medium supplemented with 0.1 mg/L NAA (90.4%). Plantlets were acclimatized on media composed of soil + sand+ organic matter+ vermiculite (2: 2: 1: 1) with 80% survival.