Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli

• Main Authors: Ong, S.T., Tan, W.S., Hassan, S.S., Mohd Lila, M.A.
• Format: Article
• Published: Taylor & Francis 2002
• Online Access: http://psasir.upm.edu.my/id/eprint/115312/; https://taylorandfrancis.com/

The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc ep...