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Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli

The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc ep...

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Main Authors: Ong, S.T., Tan, W.S., Hassan, S.S., Mohd Lila, M.A.
Format: Article
Published: Taylor & Francis 2002
Online Access:http://psasir.upm.edu.my/id/eprint/115312/
https://taylorandfrancis.com/
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spelling my.upm.eprints.1153122025-03-03T01:42:19Z http://psasir.upm.edu.my/id/eprint/115312/ Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli Ong, S.T. Tan, W.S. Hassan, S.S. Mohd Lila, M.A. The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc epitope and His-tag at its C-terminal end. The amount of the fusion protein expressed in strain SG 935 was significantly higher than the other two strains, and was detected by the anti-myc antibody, anti-His and swine anti-NiV serum. Hence, the Nfus protein produced in E. coli could serve as an alternative antigen for the detection of anti-NiV in swine. Taylor & Francis 2002 Article PeerReviewed Ong, S.T. and Tan, W.S. and Hassan, S.S. and Mohd Lila, M.A. (2002) Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli. Journal of Biochemistry, Molecular Biology and Biophysics, 6 (5). pp. 347-350. ISSN 1025-8140; eISSN: 1025-8140 https://taylorandfrancis.com/ 10.1080/1025814021000013994
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The coding region of the nucleocapsid (N) gene was amplified from the viral RNA and inserted into the bacterial expression vector, pTrcHis2, for intracellular expression in three Escherichia coli strains: TOP 10, BL 21 and SG 935. The N protein was expressed as a fusion protein containing the myc epitope and His-tag at its C-terminal end. The amount of the fusion protein expressed in strain SG 935 was significantly higher than the other two strains, and was detected by the anti-myc antibody, anti-His and swine anti-NiV serum. Hence, the Nfus protein produced in E. coli could serve as an alternative antigen for the detection of anti-NiV in swine.
format Article
author Ong, S.T.
Tan, W.S.
Hassan, S.S.
Mohd Lila, M.A.
spellingShingle Ong, S.T.
Tan, W.S.
Hassan, S.S.
Mohd Lila, M.A.
Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
author_facet Ong, S.T.
Tan, W.S.
Hassan, S.S.
Mohd Lila, M.A.
author_sort Ong, S.T.
title Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
title_short Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
title_full Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
title_fullStr Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
title_full_unstemmed Cloning and expression of the nucleocapsid protein gene of Nipah virus in different strains of Escherichia coli
title_sort cloning and expression of the nucleocapsid protein gene of nipah virus in different strains of escherichia coli
publisher Taylor & Francis
publishDate 2002
url http://psasir.upm.edu.my/id/eprint/115312/
https://taylorandfrancis.com/
_version_ 1825810730397466624
score 13.244413

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