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Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus

The major obstacle in the study of HBV has been the inability to infect either animal model system in vivo or continuous cell lines in vitro due to strict virushost range and preferential attachment of the HBV envelope proteins onto the plasma membrane of human hepatocytes. The direct involvement...

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Main Author: Wang, Suk Mei
Format: Thesis
Language:English
English
Published: 2001
Online Access:http://psasir.upm.edu.my/id/eprint/11228/1/FPSK_M_2001_13_A.pdf
http://psasir.upm.edu.my/id/eprint/11228/
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spelling my.upm.eprints.112282011-08-08T05:06:05Z http://psasir.upm.edu.my/id/eprint/11228/ Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus Wang, Suk Mei The major obstacle in the study of HBV has been the inability to infect either animal model system in vivo or continuous cell lines in vitro due to strict virushost range and preferential attachment of the HBV envelope proteins onto the plasma membrane of human hepatocytes. The direct involvement of human annexin-V, a calcium dependent phospholipid - binding protein in the initial step of HBV infection has been reported. Over the last decade, various culture conditions with exogenous soluble factors have also been exploited to enhance HBV infection in vitro. The aim of this study was to establish an in vitro cell culture system that would continuously produce sufficient HBV by episomal replication. To facilitate the penetration and internalization of HBV, the expression of annexin V (A V) was 2001-07 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/11228/1/FPSK_M_2001_13_A.pdf Wang, Suk Mei (2001) Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus. Masters thesis, Universiti Putra Malaysia. English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description The major obstacle in the study of HBV has been the inability to infect either animal model system in vivo or continuous cell lines in vitro due to strict virushost range and preferential attachment of the HBV envelope proteins onto the plasma membrane of human hepatocytes. The direct involvement of human annexin-V, a calcium dependent phospholipid - binding protein in the initial step of HBV infection has been reported. Over the last decade, various culture conditions with exogenous soluble factors have also been exploited to enhance HBV infection in vitro. The aim of this study was to establish an in vitro cell culture system that would continuously produce sufficient HBV by episomal replication. To facilitate the penetration and internalization of HBV, the expression of annexin V (A V) was
format Thesis
author Wang, Suk Mei
spellingShingle Wang, Suk Mei
Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
author_facet Wang, Suk Mei
author_sort Wang, Suk Mei
title Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
title_short Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
title_full Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
title_fullStr Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
title_full_unstemmed Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
title_sort establishment of an in vitro cell culture system for human hepatitis b virus
publishDate 2001
url http://psasir.upm.edu.my/id/eprint/11228/1/FPSK_M_2001_13_A.pdf
http://psasir.upm.edu.my/id/eprint/11228/
_version_ 1643824777507373056
score 13.18916

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