Establishment Of An In Vitro Cell Culture System For Human Hepatitis B Virus
The major obstacle in the study of HBV has been the inability to infect either animal model system in vivo or continuous cell lines in vitro due to strict virushost range and preferential attachment of the HBV envelope proteins onto the plasma membrane of human hepatocytes. The direct involvement...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English |
| Published: |
2001
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/11228/1/FPSK_M_2001_13_A.pdf http://psasir.upm.edu.my/id/eprint/11228/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The major obstacle in the study of HBV has been the inability to infect either
animal model system in vivo or continuous cell lines in vitro due to strict virushost
range and preferential attachment of the HBV envelope proteins onto the
plasma membrane of human hepatocytes. The direct involvement of human
annexin-V, a calcium dependent phospholipid - binding protein in the initial step
of HBV infection has been reported. Over the last decade, various culture
conditions with exogenous soluble factors have also been exploited to enhance
HBV infection in vitro.
The aim of this study was to establish an in vitro cell culture system that would
continuously produce sufficient HBV by episomal replication. To facilitate the
penetration and internalization of HBV, the expression of annexin V (A V) was |
|---|