Probing the substrate binding modes and catalytic mechanisms of bleg-1, a promiscuous b3 metallo-β-lactamase with glyoxalase ii properties

• Main Authors: Au, Shaw Xian, Mohd Padzil, Azyyati, Muhd Noor, Noor Dina, Matsumura, Hiroyoshi, Raja Abdul Rahman, Raja Noor Zaliha, Normi, Yahaya M.
• Format: Article
• Language: English
• Published: Public Library of Science 2023
• Online Access: http://psasir.upm.edu.my/id/eprint/110023/1/journal.pone.0291012.pdf; http://psasir.upm.edu.my/id/eprint/110023/; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0291012

BLEG-1 from <jats:italic>Bacillus lehensis</jats:italic> G1 is an evolutionary divergent B3 metallo-β-lactamase (MBL) that exhibited both β-lactamase and glyoxalase II (GLXII) activities. Sequence, phylogeny, biochemical and structural relatedness of BLEG-1 to B3 MBL and...