Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize

The present work investigated the potential of fungal species from grain maize farms in Malaysia as antagonists against the indigenous mycotoxigenic fungal species and their subsequent mycotoxin production. Dual-culture assay was conducted on grain maize agar (GMA) with 12 strains of potential funga...

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Main Authors: Yazid, Siti Nur Ezzati, Tajudin, Nur Izzah, Razman, Nur Aina Aribah, Selamat, Jinap, Ismail, Siti Izera, Sanny, Maimunah, Samsudin, Nik Iskandar Putra
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Published: Springer 2023
Online Access:http://psasir.upm.edu.my/id/eprint/109052/
https://link.springer.com/article/10.1007/s12550-023-00484-4?error=cookies_not_supported&code=37ca18f3-4f41-43ff-a70f-04360f271a45
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spelling my.upm.eprints.1090522024-10-14T03:50:20Z http://psasir.upm.edu.my/id/eprint/109052/ Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize Yazid, Siti Nur Ezzati Tajudin, Nur Izzah Razman, Nur Aina Aribah Selamat, Jinap Ismail, Siti Izera Sanny, Maimunah Samsudin, Nik Iskandar Putra The present work investigated the potential of fungal species from grain maize farms in Malaysia as antagonists against the indigenous mycotoxigenic fungal species and their subsequent mycotoxin production. Dual-culture assay was conducted on grain maize agar (GMA) with 12 strains of potential fungal antagonists namely Bjerkandra adusta, Penicillium janthinellum, Schizophyllum commune, Trametes cubensis, Trichoderma asperelloides, Trichoderma asperellum, Trichoderma harzianum, and Trichoderma yunnanense against seven mycotoxigenic strains namely Aspergillus flavus, Aspergillus niger, Fusarium verticillioides, and Fusarium proliferatum producing aflatoxins, ochratoxin A, and fumonisins, respectively. Based on fungal growth inhibition, Trichoderma spp. showed the highest inhibitory activity (73–100% PIRG, Percentage Inhibition of Radial Growth; 28/0 ID, Index of Dominance) against the tested mycotoxigenic strains. Besides, B. adusta and Tra. cubensis showed inhibitory activity against some of the tested mycotoxigenic strains. All fungal antagonists showed varying degrees of mycotoxin reduction. Aflatoxin B1 produced by A. flavus was mainly reduced by P. janthinellum, Tra. cubensis, and B. adusta to 0 ng/g. Ochratoxin A produced by A. niger was mainly reduced by Tri. harzianum and Tri. asperellum to 0 ng/g. Fumonisin B1 and FB2 produced by F. verticillioides was mainly reduced by Tri. harzianum, Tri. asperelloides, and Tri. asperellum to 59.4 and 0 µg/g, respectively. Fumonisin B1 and FB2 produced by F. proliferatum were mainly reduced by Tri. asperelloides and Tri. harzianum to 244.2 and 0 µg/g, respectively. This is the first study that reports on the efficacy of Tri. asperelloides against FB1, FB2, and OTA, P. janthinellum against AFB1, and Tra. cubensis against AFB1. Springer 2023-08 Article PeerReviewed Yazid, Siti Nur Ezzati and Tajudin, Nur Izzah and Razman, Nur Aina Aribah and Selamat, Jinap and Ismail, Siti Izera and Sanny, Maimunah and Samsudin, Nik Iskandar Putra (2023) Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize. Mycotoxin Research, 39 (3). pp. 177-192. ISSN 0178-7888; ESSN: 1867-1632 https://link.springer.com/article/10.1007/s12550-023-00484-4?error=cookies_not_supported&code=37ca18f3-4f41-43ff-a70f-04360f271a45 10.1007/s12550-023-00484-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present work investigated the potential of fungal species from grain maize farms in Malaysia as antagonists against the indigenous mycotoxigenic fungal species and their subsequent mycotoxin production. Dual-culture assay was conducted on grain maize agar (GMA) with 12 strains of potential fungal antagonists namely Bjerkandra adusta, Penicillium janthinellum, Schizophyllum commune, Trametes cubensis, Trichoderma asperelloides, Trichoderma asperellum, Trichoderma harzianum, and Trichoderma yunnanense against seven mycotoxigenic strains namely Aspergillus flavus, Aspergillus niger, Fusarium verticillioides, and Fusarium proliferatum producing aflatoxins, ochratoxin A, and fumonisins, respectively. Based on fungal growth inhibition, Trichoderma spp. showed the highest inhibitory activity (73–100% PIRG, Percentage Inhibition of Radial Growth; 28/0 ID, Index of Dominance) against the tested mycotoxigenic strains. Besides, B. adusta and Tra. cubensis showed inhibitory activity against some of the tested mycotoxigenic strains. All fungal antagonists showed varying degrees of mycotoxin reduction. Aflatoxin B1 produced by A. flavus was mainly reduced by P. janthinellum, Tra. cubensis, and B. adusta to 0 ng/g. Ochratoxin A produced by A. niger was mainly reduced by Tri. harzianum and Tri. asperellum to 0 ng/g. Fumonisin B1 and FB2 produced by F. verticillioides was mainly reduced by Tri. harzianum, Tri. asperelloides, and Tri. asperellum to 59.4 and 0 µg/g, respectively. Fumonisin B1 and FB2 produced by F. proliferatum were mainly reduced by Tri. asperelloides and Tri. harzianum to 244.2 and 0 µg/g, respectively. This is the first study that reports on the efficacy of Tri. asperelloides against FB1, FB2, and OTA, P. janthinellum against AFB1, and Tra. cubensis against AFB1.
format Article
author Yazid, Siti Nur Ezzati
Tajudin, Nur Izzah
Razman, Nur Aina Aribah
Selamat, Jinap
Ismail, Siti Izera
Sanny, Maimunah
Samsudin, Nik Iskandar Putra
spellingShingle Yazid, Siti Nur Ezzati
Tajudin, Nur Izzah
Razman, Nur Aina Aribah
Selamat, Jinap
Ismail, Siti Izera
Sanny, Maimunah
Samsudin, Nik Iskandar Putra
Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
author_facet Yazid, Siti Nur Ezzati
Tajudin, Nur Izzah
Razman, Nur Aina Aribah
Selamat, Jinap
Ismail, Siti Izera
Sanny, Maimunah
Samsudin, Nik Iskandar Putra
author_sort Yazid, Siti Nur Ezzati
title Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
title_short Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
title_full Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
title_fullStr Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
title_full_unstemmed Mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
title_sort mycotoxigenic fungal growth inhibition and multi‑mycotoxin reduction of potential biological control agents indigenous to grain maize
publisher Springer
publishDate 2023
url http://psasir.upm.edu.my/id/eprint/109052/
https://link.springer.com/article/10.1007/s12550-023-00484-4?error=cookies_not_supported&code=37ca18f3-4f41-43ff-a70f-04360f271a45
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