Multiresidue analysis of pesticides in fruits and vegetables using solid-phase extraction and gas chromatographic methods

• Main Authors: Lal A., Tan G., Chai M.
• Other Authors: 24172476100
• Format: Article
• Published: Japan Society for Analytical Chemistry 2023
• Subjects: Chromatography, Gas; Food Analysis; Food Contamination; Fruit; Pesticides; Reproducibility of Results; Sensitivity and Specificity; Solid Phase Extraction; Time Factors; Vegetables; Acetone; Chromatographic analysis; Fruits; Gas chromatography; Hexane; Insecticides; Phase separation; Solvents; pesticide; Analytical procedure; Chromatographic methods; Fruits and vegetables; Internal standards; Limit of detection; Limit of quantifications; Multi-residue analysis; Solid-phase extraction; article; chemistry; food analysis; food contamination; fruit; gas chromatography; instrumentation; methodology; reproducibility; sensitivity and specificity; solid phase extraction; time; vegetable; Extraction

A new extraction and cleanup procedure with gas chromatography was developed for the sensitive determination of acephate, dimethoate, malathion, diazinon, quinalphos, chlorpyrifos, profenofos, ?-endosulfan, ?-endosulfan, chlorothalonil and carbaryl using 1-chloro-4-fluorobenzene as an internal standard in fruits and vegetables. Several extracting and eluting solvents for solid-phase extraction were investigated. The overall extracting solvent with a mixture of acetone:ethyl acetate:hexane (10:80:10, v/v/v) and a eluting solvent of 5% acetone in hexane used with the RPC18 cartridge gave the best recovery for all of the investigated pesticides, and minimized the interference from co-extractants. Under the optimal extraction and clean-up conditions, recoveries of 85 - 99% with RSD < 5.0% (n = 3) for most of the pesticides at the 0.02-0.5 mg/kg level were obtained. The limit of detection was between 0.005-0.01 mg/kg and the limit of quantification was 0.01 mg/kg. This analytical procedure was characterized with high accuracy and acceptable sensitivity to meet requirements for monitoring pesticides in crops. 2008 � The Japan Society for Analytical Chemistry.